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Details
Posted: 16-Dec-22
Location: Davis, California
Type: Full-time
Salary: Open
Internal Number: 3729496
Junior/Assistant Specialist - Loots Lab
Histopathology
The Department of Orthopaedic Surgery at UC Davis Health, Loots Lab, has an opening for a histopathology specialist to participate in biomedical research conducting histopathology, immunohistochemistry on tissue sections, and cellular preparations of homogenized tissues for sorting or sequencing applications. The position will serve in the Specialist series with a Junior or Assistant rank. The candidate will work in a highly integrated environment as part of a multi-disciplinary team to develop, optimize, characterize, and validate methods for assessing protein and gene expression using 10X Genomics and CosMx NanoString technology platforms in the context of several models of human diseases including cancer, post-traumatic osteoarthritis and osteoporosis. The work combines basic histology techniques including, tissue harvesting and microdissection, fixation, dehydration, sectioning, staining, imaging and image processing. Ensures tissue and cytology specimens are properly processed for histopathology, immunohistochemistry, in situ hybridization or spatial transcriptomics. This position will also include cellular analysis of tissues of interest via flow cytometry and/or single cell sequencing.
The position will work under the direction of Dr. Gabriela Loots in the Department of Orthopaedic Surgery.
This recruitment is conducted at the Junior or Assistant rank. The resulting hire will be at the Junior or Assistant rank, regardless of the proposed appointee's qualifications.
Essential Duties
- Assist with tissue collection, fixation and embedding. Develop protocols and procedures for both soft (lung, tumors, etc) and hard tissues (bones, cartilage) using different fixation techniques (paraffin and OCT).
- Generate tissue sections by operating microtome, cryotome, and/or vibrotome, depending on tissue type and analyses required by active projects.
- Stain sectioned tissue with various histological stains (Safranin O, Trichrome, H&E Von Kossa), image tissue, annotate images with relevant information.
- Optimize antibody selection for use in immunohistochemical (IHC) staining of fixed and sectioned tissues, process protein/gene expression analysis using CosMX Nanostring and Visium 10X instrument. Conduct IHCs and image protein expression. Troubleshoot initial results and optimize multi-color imaging to visualize several different proteins in the same tissue section.
- Optimize and prepare tissue sections for spatial transcriptomics and other 10X genomics technologies
- Optimize tissue digests for single cell sequencing or fluorescently activated cell sorting (FACS)
- Prepare single cell suspensions for single cell RNA sequencing
- Maintain an accurate and detailed laboratory journal.
- Ensure all experiments are performed within ES&H safety guidelines and that waste from all experiments is characterized and disposed of properly.
- Occasional weekend work and/or additional hours during the week may be required to follow through on ongoing experiments.
- Perform other duties as assigned.
POSITION DESCRIPTION
Research in Specialized Areas (95% effort)
- Participate in biomedical research conducting histopathology, immunohistochemistry on tissue sections, and cellular preparations of homogenized tissues for sorting or sequencing applications.
Professional Competence and Activity (3%)
- May participate in appropriate professional/technical societies or groups and other educational and research organizations.
- May review research proposals, journal manuscripts, and publications related to area of expertise.
University and Public Service (2% effort)
- May maintain liaison and respond to the needs of various industry organizations, state and federal agencies, and other external groups on issues related to area of expertise.
- May participate in activities of meetings/committees within the department, college, campus and other University entities, as appropriate.
Number of positions Available: 1
To apply, visit https://recruit.ucdavis.edu/JPF05414
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About University of California Davis
UC Davis is the home of the Aggies — go-getters, change makers and problem solvers who make their mark at one of the top public universities in the United States. Since we first opened in 1908, we’ve been known for standout academics, sustainability and Aggie Pride as well as valuing the Northern California lifestyle. These themes are woven into our 100-plus-year history and our reputation for solving problems related to food, health, the environment and society.Our 5,300-acre campus is in the city of Davis, a vibrant college town of about 68,000 located in Yolo County. The state capital is 20 minutes away, and world-class destinations such as the San Francisco Bay Area, Lake Tahoe and the Napa Valley are within a two-hour drive.
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https://careers.ors.org/jobs/17925118/junior-assistant-specialist-loots-lab
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