The Center for Cellular and Molecular Engineering (CCME) of the University of Pittsburgh is seeking a Research Technician.
As a Research Technician in this lab, the incumbent will be a part of an exciting state-of-art internationally recognized tissue and cellular engineering research program in the area of musculoskeletal research, with a specific focus on cartilage and bone. The position is supported by a funded project and the Research Technician will work under the direct supervision of the senior scientists on the project. The incumbent will manage cell and tissue cultures and assess cell proliferation, differentiation, and metabolism using RT_PCR, biochemistry, histology, immunohistochemistry, and immunofluorescence (from sample preparation, to sectioning, to staining, etc). A major focus of the research will be on the development of 3D microphysiological constructs maintained in unique bioreactor systems and scaffolds developed in the lab.
The Research Technician is expected to maintain accurate records of laboratory procedures and results. Operating and maintaining sensitive laboratory equipment is also part of the job. The prompt communication of results, issues, and needs with team members is necessary. The incumbent must have a good knowledge of laboratory skills and a high level of self-sufficiency. The capacity to work independently (including the design and execution of experiments) with some direction from senior scientists is required. Experience in literature searches, identification of existing protocols and establishment/adaptation of protocol for specific cell culture conditions are expected as well as modifying standard procedures and methods to address the specific conditions of this project. Experience with mammalian cells culture is required. Experience in using optical microscopes in bright field mode and in fluorescence mode is required. Experience in either RT PCR or tissue histology/immunohistochemistry is required.
This project requires the use of biochemical, histo/immune-histochemical and molecular techniques including, but not limited to, MTT, DMMB, alkaline phosphatase, TRAP, and hydroxyproline assays, DNA/RNA preparation and handling, RT PCR, western blotting and ELISAs, microscopy, basic histological staining, and immunohistochemistry (including immunofluorescence). Hence, the capacity to perform laboratory procedures and tests is expected. The ability to use the Office suite, including Excel, is required, as is the capacity to perform the basic calculations for dilutions, standard curves, and quantification of data obtained by assays such as RT PCR or colorimetric assays. Multi-tasking several experiments simultaneously and efficient time management is necessary. Many of the responsibilities described above will be self-initiated and require a high level of vigilance. Meticulous work habits, dependability, and good communication skills are required. Two years experience of wet lab work are required.
Cover letter should describe research experience, long-term career goals, and why you would be a good fit for this position.
This is position is subject to funding.
Internal Number: 154420
About University of Pittsburgh
Founded in 1787, the same year the U.S. Constitution was signed, the University of Pittsburgh is one of the oldest institutions of higher education in the United States. One of three state-related research universities in Pennsylvania, Pitt is a member of the Association of American Universities (AAU), which comprises 62 preeminent doctorate-granting research institutions in North America.
Pitt faculty members have expanded knowledge in the humanities and sciences, earning such prestigious honors as the National Medal of Science, the MacArthur Foundation’s “genius” grant, the Lasker-DeBakey Clinical Medical Research Award, and election to the National Academy of Sciences and the Institute of Medicine. Pitt scientists have defeated polio, unlocked the secrets of DNA, lead the world in organ transplantation, and pioneered TV and heavier-than-air flight, among numerous other accomplishments.
Pitt students have earned Rhodes, Goldwater, Marshall, and Truman Scholarships, among other highly competitive national and international scholarships.
Alumni have pioneered MRI and TV, won Nobel and Pulitzer Prizes, led corporations and universities, served in government and the military..., conquered Hollywood and The New York Times bestsellers list, and won Super Bowls and NBA championships.